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Description of Individual Course Units
| Course Unit Code | Course Unit Title | Type of Course Unit | Year of Study | Semester | Number of ECTS Credits |
|---|---|---|---|---|---|
| BKM204 | BASIC TECHNIQUES IN BIOCHEMISTRY | 2 | 4 | 5,00 |
Level of Course Unit
Lisans
Language of Instruction
Objectives of the Course
Name of Lecturer(s)
Learning Outcomes
| 1 | Be able to use the basic theoretical and practical issues involved in the separation and analysis of biological molecules |
| 2 | Gain an ability to work effectively in laboratory environment and to use biochemical instrumentation and procedures |
| 3 | Gain an ability to think critically and to analyze biochemical problems |
| 4 | Gain an ability to work with people in teams to solve biochemical problems and enhance independent work capability |
| 5 | Be able to interpret data, carry out experiments and evaluate results |
| 6 | Gain an ability to understand scientific literature searching procedures |
| 7 | Gain an understanding on preparation of laboratory notebook and report preparation, data analysis and the use of computers in experiments |
| 8 | Gain an ability to communicate effectively, both orally and in writing |
| 9 | Be able to use techniques, skills and tools necessary for biochemistry |
| 10 | Be able to understand the basics of laboratory safety and biosafety |
| 11 | Be able to understand scientific/academic honesty and ethical issues |
Mode of Delivery
Prerequisities and Co-Requisities
Recommended Optional Programme Components
[Yok]
Content of the Course
Weekly Detailed Course Contents
| Week | Subjects (Theoretical) | Teaching Methods | Preparatory |
|---|---|---|---|
| 1 | Water as a reagent, Water Purification and types of water for experiments in Biochemistry. Laboratory safety and Biosafety Rules, Laboratory notebook and Preparation of reports, Errors, Graphing, | ||
| 2 | Biological sample preparation opportunities and limitings. Choice of starting material, Cell disruption/homogenization: Choice of method, Efficiency of disruptive method. | ||
| 3 | Mechanical Methods; Homogenizators, Blenders, grinders, agitation with abbresives, liquid and solid extrusion, ultrasonication Chemical Methods ; heat treatment, freeze-thaw, desiccation, osmotic shock, lytic enzymes, alkali treatment, detergents, etc. | ||
| 4 | Centrifugation; Theory, Classification, Types of Centrifuges, Differential centrifugation, Density gradient centrifugation, Membrane filtration techniques | ||
| 5 | Concentration of the extract; Ultrafiltration, Lyophilization, Precipitation techniques( salting-out, salting-in, isoelectric, organic solvents and polymers) | ||
| 6 | Chromatography, Theory, applications, types of Chromatography, Paper and Thin Layer Chromatography, Basic principles and theory of liquid chromatography, | ||
| 7 | Chromatographic techniques; Ion-exchange chromatography. Hydrophobic interaction chromatography | ||
| 8 | Mid-term Exam | ||
| 9 | Chromatographic techniques; Gel Filtration Affinity Chromatography; Biospesific, Lectin affinity, Dye-ligand, IMAC, Covalent Affinity | ||
| 10 | Electrophoretic techniques; Principles of electrophoresis; PAGE, SDS-PAGE, IEF, 2D-PAGE,. Blotting, Imaging, DNA Electrophoresis | ||
| 11 | Immunological Techniques ( Immuno precipitation, Immunoblotting, ELISA) | ||
| 12 | HPLC and its applications | ||
| 13 | Integration and comparison of the methods | ||
| 14 | Tutorial problem discussion | ||
| 15 | Tutorial problem discussion | ||
| 16 | Final Exam |
Text Book / Material / Recommended Resources
Planned Learning Activities and Teaching Methods
Assessment Methods and Criteria
| Term (or Year) Learning Activities | Quantity | Weight |
|---|---|---|
| Midterm Examination | 1 | 100 |
| Total | 100 | |
| End Of Term (or Year) Learning Activities | Quantity | Weight |
| Final Examination | 1 | 100 |
| Total | 100 | |
| Term (or Year) Learning Activities | 40 | |
| End Of Term (or Year) Learning Activities | 60 | |
Work Pacement(s)
Workload Calculation
| Activities | Number | Time (hours) | Total Work Load (hours) |
|---|---|---|---|
| Midterm Examination | 1 | 2 | 2 |
| Final Examination | 1 | 2 | 2 |
| Attending Lectures | 14 | 2 | 28 |
| Tutorial | 6 | 2 | 12 |
| Discussion | 4 | 3 | 12 |
| Question-Answer | 6 | 2 | 12 |
| Team/Group Work | 6 | 4 | 24 |
| Individual Study for Mid term Examination | 1 | 15 | 15 |
| Individual Study for Final Examination | 1 | 20 | 20 |
| Reading | 5 | 4 | 20 |
| Total Work Load (hours) | 147 | ||
Contribution of Learning Outcomes to Programme Outcomes
| PO 1 | PO 2 | PO 3 | PO 4 | PO 5 | PO 6 | PO 7 | PO 8 | PO 9 | PO 10 | PO 11 | PO 12 | PO 13 | PO 14 | PO 15 | |
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* Contribution Level: 1 Very low 2 Low 3 Medium 4 High 5 Very High