BACK
Description of Individual Course Units
| Course Unit Code | Course Unit Title | Type of Course Unit | Year of Study | Semester | Number of ECTS Credits |
|---|---|---|---|---|---|
| 9301116342013 | Light Microscopy Preparation Techniques (Immunohistochemistry-Immunofluorescence) II | 1 | 1 | 4,00 |
Level of Course Unit
Third Cycle
Language of Instruction
Turkish
Objectives of the Course
To learn principles of light microscopic immunohistochemistry
Name of Lecturer(s)
Learning Outcomes
| 1 | Light microscopy, to gain the ability to use light microscopes |
| 2 | To learn about light microscopic equipment |
| 3 | To prepare chemicals in immunohistochemistry, to apply fixation technics |
| 4 | To choose appropriate fixatives |
| 5 | Dehydration and clearing |
| 6 | Embedding in paraffine |
| 7 | Sectioning via microtome |
| 8 | Parafine block preparation and embedding tissues |
| 9 | Sectioning via microtome and placing tissue on slides |
| 10 | Choosing the appropriate stain |
| 11 | Applying the appropriate staining technique |
| 12 | Photography through the microscope |
| 13 | Problem solving through technical steps |
| 14 | To understand,apply and evaluate through all steps about immunohistochemical methodology |
Mode of Delivery
Face to Face
Prerequisities and Co-Requisities
None
Recommended Optional Programme Components
None
Content of the Course
Laboratory equipment, preparation of the fixatives, smapling fixation, dehydration and clearing of tissue samples, embedding in paraffine sectioning via microtome, immunohistochemical staining procedures, light microscopic investigation, photography and evaluation.
Weekly Detailed Course Contents
| Week | Subjects (Theoretical) | Practice, Laboratory / Laboratuvar | Teaching Methods | Preparatory |
|---|---|---|---|---|
| 1 | Immunohistochemistry, general principles in immunocytochemistry. Steps in tissue preparation, equipment in histology laboratory | Immunohistochemistry, general principles in immunocytochemistry. Steps in tissue preparation, equipment in histology laboratory | ||
| 2 | Structure of the immunoglobulin, immunohistochemical staining methods, fixation methods,and fixative solutions, choosing the appropriate fixatives and preparation | Structure of the immunoglobulin, immunohistochemical staining methods, fixation methods,and fixative solutions, choosing the appropriate fixatives and preparation | ||
| 3 | Perfusion of experimental animals, tissue sampling and immersion | Perfusion of experimental animals, tissue sampling and immersion | ||
| 4 | Dehydration | Dehydration | ||
| 5 | Clearing | Clearing | ||
| 6 | Liquid parafine steps and embedding in parafine (Block preparation) | Liquid parafine steps and embedding in parafine (Block preparation) | ||
| 7 | Sectioning via microtome and slide mounting | Sectioning via microtome and slide mounting | ||
| 8 | Avidin-biotin complex method | Avidin-biotin complex method | ||
| 9 | Preparation of DAB, antibody dilution and preparation | Preparation of DAB, antibody dilution and preparation | ||
| 10 | Immunohistochemical staining | Immunohistochemical staining | ||
| 11 | Preparation of trypsin, hydrogen peroxyde and phosphate buffer solutions | Preparation of trypsin, hydrogen peroxyde and phosphate buffer solutions | ||
| 12 | Immunohistochemical staining | Immunohistochemical staining | ||
| 13 | Immunohistochemical staining | Immunohistochemical staining | ||
| 14 | Evaluation of prepared tissue slides | Evaluation of prepared tissue slides | ||
| 15 | Photography Evaluation of prepared tissue slides and photography | Photography Evaluation of prepared tissue slides and photography | ||
| 16 | General evaluation of laboratory studies Final presentation | General evaluation of laboratory studies Final presentation |
Text Book / Material / Recommended Resources
Histolojik Boyama Teknikleri: R. Demir. Temel Histoloji . Junqueira, Carneiro. Çev.ed.: S. Solakoğlu, Y. Aytekin
Planned Learning Activities and Teaching Methods
Assessment Methods and Criteria
| Term (or Year) Learning Activities | Quantity | Weight |
|---|---|---|
| Laboratory Midterm Examination | 1 | 100 |
| Total | 100 | |
| End Of Term (or Year) Learning Activities | Quantity | Weight |
| Final Examination | 1 | 100 |
| Total | 100 | |
| Term (or Year) Learning Activities | 40 | |
| End Of Term (or Year) Learning Activities | 60 | |
Work Pacement(s)
None
Workload Calculation
| Activities | Number | Time (hours) | Total Work Load (hours) |
|---|---|---|---|
| Laboratory | 14 | 2 | 28 |
| Experiment | 14 | 2 | 28 |
| Observation | 14 | 1 | 14 |
| Brain Storming | 14 | 1 | 14 |
| Report Preparation | 1 | 20 | 20 |
| Report Presentation | 1 | 1 | 1 |
| Seminar | 2 | 1 | 2 |
| Self Study | 14 | 1 | 14 |
| Total Work Load (hours) | 121 | ||
Contribution of Learning Outcomes to Programme Outcomes
| PO 1 | PO 2 | PO 3 | PO 4 | PO 5 | PO 6 | PO 7 | PO 8 | PO 9 | PO 10 | |
| LO 1 | 4 | 4 | 4 | 4 | 4 | 4 | 4 | 4 | 4 | 4 |
| LO 2 | 5 | 3 | 4 | 5 | 4 | 4 | 5 | 4 | 4 | 4 |
| LO 3 | 3 | 4 | 4 | 3 | 4 | 5 | 4 | 3 | 3 | 4 |
| LO 4 | 4 | 5 | 5 | 5 | 5 | 4 | 4 | 4 | 4 | 4 |
| LO 5 | 3 | 4 | 4 | 4 | 4 | 3 | 4 | 4 | 3 | 5 |
| LO 6 | 5 | 5 | 4 | 5 | 5 | 3 | 4 | 4 | 4 | 4 |
| LO 7 | 4 | 4 | 3 | 4 | 4 | 4 | 5 | 4 | 4 | 5 |
| LO 8 | 3 | 4 | 5 | 4 | 5 | 4 | 5 | 4 | 4 | 4 |
| LO 9 | 4 | 3 | 4 | 4 | 4 | 5 | 4 | 3 | 4 | 4 |
| LO 10 | 3 | 4 | 3 | 4 | 3 | 4 | 4 | 4 | 3 | 5 |
| LO 11 | 4 | 4 | 5 | 3 | 5 | 3 | 5 | 4 | 3 | 3 |
| LO 12 | 4 | 5 | 2 | 5 | 4 | 4 | 4 | 3 | 4 | 5 |
| LO 13 | 5 | 3 | 5 | 4 | 5 | 5 | 4 | 5 | 4 | 4 |
| LO 14 | 5 | 5 | 3 | 3 | 4 | 4 | 4 | 4 | 4 | 5 |
* Contribution Level: 1 Very low 2 Low 3 Medium 4 High 5 Very High